differentiation of m. scrofulaceum from related mycobacterial species by double-diffusion technique

نویسندگان

آذر خسروی

azar d. khosravi

john l stanford

چکیده

isolation and differentiation of various species of mycobacteria are predominantly based on conventional, cultural and biochemical tests. among the non-tuberculous mycobacteria, these tests are unable to differentiate m. scrofulaceum from m. avium-intracellulare (mai) because of their identical biochemical characteristics. for this reason, one hundred mycobacterial strains suspected to be m. scrofulaceum, which had identical preliminary cultural and biochemical tests, were investigated by immunodiffusion technique. the standard sonicated antigens of m. scrofulaceum (22, 159, 1281) were used to sensitize rabbits and to produce specific antisera. the method was performed in plastic petri dishes containing agar and sodium azide. antiserum was placed in the central well of the agar plate and the homologous ultrasonicates were in the peripheral wells. the results were observed one week later and the mycobacterial strains were divided according to the number of produced precipitation lines. four different groups were identified on the basis of identical reaction with three antisera (three groups were m. scrofulaceum). group 1 produced identical precipitation lines with antisera 22 and 1281, group 2 with antisera 22, 159 and 1281 and group 3 with antiserum 1281. the present study showed that the technique was able to differentiate m. scrofulaceum from mai and was also able to identify the bacterium as distinct species. moreover, by using different specific antisera, it is possible to determine different serotypes of m. scrofulaceum.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Differentiation of M. scrofulaceum from Related Mycobacterial Species by Double-Diffusion Technique

Isolation and differentiation of various species of mycobacteria are predominantly based on conventional, cultural and biochemical tests. Among the non-tuberculous mycobacteria, these tests are unable to differentiate M. scrofulaceum from M. avium-intracellulare (MAI) because of their identical biochemical characteristics. For this reason, one hundred mycobacterial strains suspected to be M. ...

متن کامل

Differentiation of M. scrofulaceum from Related Mycobacterial Species by Double-Diffusion Technique

Isolation and differentiation of various species of mycobacteria are predominantly based on conventional, cultural and biochemical tests. Among the non-tuberculous mycobacteria, these tests are unable to differentiate M. scrofulaceum from M. avium-intracellulare (MAI) because of their identical biochemical characteristics. For this reason, one hundred mycobacterial strains suspected to be M. sc...

متن کامل

Rapid lysis technique for mycobacterial species.

The lysis of mycobacterial cells typically has been difficult and time-consuming. We report a method for the physical rupture of Mycobacterium paratuberculosis and several other members of the family Mycobacteriaceae using a Mini-Beadbeater cell disrupter (Biospec Products; Bartlesville, Okla.) and zirconium beads, a process which yields DNA and RNA of high molecular weight and in greater quant...

متن کامل

Differentiation of two closely related species of the genus Empoasca (Hem.: Cicadellidae)

پیش‌تر، گونه‌ای زنجرک از مزارع چغندرقند استان اصفهان به اشتباه به نام Empoasca decipiens (Paoli) گزارش شده است که در این نوشتار نام صحیح آن به‌صورت Empoasca meridiana Zachvatkin اعلام می‌گردد. گونه‌ی اخیر دارای سینونیم شناخته‌تری به نام Empoasca punjabensis Singh-Pruthi می‌باشد. گونه‌های آسیایی خویشاوند گونه‌ی E. decipiens، براساس شکل پیگوفر (pygofer) ژنیتالیای نر ازهم تفکیک می‌گردند، به‌طوری‌ک...

متن کامل

Differentiation between Mycobacterium bovis BCG-vaccinated and M. bovis-infected cattle by using recombinant mycobacterial antigens.

Tuberculosis continues to be a worldwide problem for both humans and animals. The development of tests to differentiate between infection with Mycobacterium tuberculosis or Mycobacterium bovis and vaccination with M. bovis BCG could greatly assist in the diagnosis of early infection as well as enhance the use of tuberculosis vaccines on a wider scale. Recombinant forms of four major secreted pr...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian biomedical journal

جلد ۴، شماره ۴، صفحات ۱۱۳-۱۱۶

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023